GenomicsWorkflow

Single-Cell RNA-seq Workflow

A six-stage single-cell sequencing workflow from tissue dissociation through UMAP clustering.

Discipline: Genomics
Input mode: Text To Image
Style: Publication clean
Canvas ratio: 16:9

Single-Cell RNA-seq Workflow template preview

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A horizontal six-panel scientific workflow figure tracing a single-cell RNA-seq pipeline left to right, with numbered stage badges (1 to 6) and curved arrows connecting panels. Panel 1, Tissue Dissociation, shows an excised organ being enzymatically digested into a single-cell suspension labeled "viable cells, >85% viability". Panel 2, Droplet Capture, depicts a microfluidic chip co-encapsulating cells and barcoded gel beads into oil droplets ("GEMs, ~10,000 cells"). Panel 3, Reverse Transcription, shows in-droplet mRNA capture with cell barcode plus UMI plus poly-T primer generating cDNA. Panel 4, Library Prep and Sequencing, illustrates amplified libraries on an Illumina NovaSeq flow cell ("paired-end, 50k reads/cell"). Panel 5, Count Matrix, renders a genes-by-cells heatmap grid with axis labels "Genes" and "Cell barcodes". Panel 6, UMAP Clustering, plots colored cell clusters labeled T cells, B cells, Monocytes, NK cells, Dendritic cells, with UMAP-1 and UMAP-2 axes. Use a clean publication-quality style, muted palette, tidy alignment, legible sans-serif labels, and a white background.

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Single-Cell RNA-seq Workflow Scientific Figure Template | LabFig